For httpd.conf ( /usr/local/apache/conf – if you compile by source OR /etc/httpd/conf/httpd -: if you compile by yum) ScriptAlias /cgi-bin/ "/usr/local/apache/cgi-bin/" If you want to run cgi script from under your domain , example , www.fosiul.com/cgi-bin/test.cgi , do as bellow <VirtualHost *:80> ServerAdmin fosiul@example.co.uk DocumentRoot /usr/local/apache/htdocs/example/ ServerName www.example.co.uk ServerAlias example.co.uk ……………………………….. ……………………………….. <Directory "/usr/local/apache/htdocs/example/"> [...]